Metabolomics-Plattform

activities

The METABO platform is dedicated to the study of small molecules, specifically polar metabolites and lipids. Our work focuses on applying and advancing metabolomics approaches within biomedical research fields, particularly cancer research. Beyond utilising state-of-the-art metabolomics, we actively develop and implement novel analytical methods to expand metabolome coverage and support emerging research directions.

We conduct metabolomic analyses on a variety of sample types, including body fluids, cell extracts, and tissues. Our platform has established a diverse portfolio of methods that cover key metabolic pathways and offer extensive metabolome coverage. We specialise in mass spectrometry-based metabolomics and lipidomics, supported by two LC-MS (liquid chromatography–mass spectrometry) instruments utilising Orbitrap technology (Thermo):

• Vanquish Flex LC coupled to a Q Exactive Plus Orbitrap
  • Used for routine detection of small polar metabolites, including glycolytic and TCA cycle intermediates, amino acids, and their ¹³C-labeled isotopologues.
• Vanquish Horizon LC coupled to an Exploris 480
  • Supports a wide range of applications, including phospholipids quantification, short-chain fatty acids quantification, deuterium tracing experiments, and the quantification of polar metabolites and their ¹³C-labeled counterparts.

Our data processing capabilities include both targeted and untargeted approaches, ensuring robust and comprehensive metabolomic analysis.

Finally, fostering the next generation of researchers is a core mission of our platform. To this end, we provide comprehensive theoretical and practical training in metabolomics.

Nathalie
Legrave

Contact

Head of Metabolomics Platform